AB0078 LIPID IMMOBILIZATION AS A METHOD TO OBTAIN ANTIGENIC NANO-OBJECTS

Objectives: Objective of the study is to research effect emulsion polymerization on active sites cardiolipin antigen determinant in antiphospholipid syndrome (APS) patients with systemic lupus erythematosus (SLE). Methods: Having integrated nanoobjects we developed immobilized magnetocontrollable nanosystems and put them an evaluation test. The are polyacrylamide granules a built antigen. To obtain stable multi–use biopharmaceuticals targeted properties (shape, particle diameter, pore size, density) used modified version method using carrier gel. This permitted greater sorptive capacity, preserving maximum native state, opened up possibility controllable modification nanoobjects. Cardiolipin was as question. Results: Following described above performed sorption anticardiolipin antibodies from blood plasma SLE who showed clinical presentations syndrome. Blood serum 10 apparently healthy individuals served control. level determined before after by indirect solid phase immunoenzyme method. In eluate estimated total protein Lowry vitro testing that obtained based could effectively remove whole APS achieve values (before 0.328 ± 0.028; 0.059 0.017; p<0.001; capacity 8.00 0.390 mg/ml). Conclusion: consideration hydrophobic hydrophilic lipid molecules permits obtaining modifying biomolecules certain properties, controlled fashion. Disclosure Interests: None declared